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Sanger sequencing, also known as the dideoxy chain termination method, is a pioneering technique for determining the order of nucleotides (A, C, G, T) in a DNA molecule. Developed by Frederick Sanger and his colleagues in 1977, it paved the way for modern DNA sequencing methods.
A researcher wants to verify the sequence of a gene they have amplified using PCR. They can perform Sanger sequencing on the PCR product. By analyzing the banding pattern on the gel, they can confirm if the amplified fragment matches the expected sequence and identify any potential errors introduced during PCR.
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